منابع مشابه
Thin Sectioning: Details of Techniques
The study of cytological fine structure with the electron microscope, has as an important prerequisite, the ability to cut consistently good quality thin sections. Satisfactory techniques for doing this are well known in many laboratories and an excellent review of the subject has recently been published by Farquhar (1). However, some of the details of the practice of microtomy are not in the l...
متن کاملThin Sectioning of Slice Preparations for Immunohistochemistry
Many investigations in neuroscience, as well as other disciplines, involve studying small, yet macroscopic pieces or sections of tissue that have been preserved, freshly removed, or excised but kept viable, as in slice preparations of brain tissue. Subsequent microscopic studies of this material can be challenging, as the tissue samples may be difficult to handle. Demonstrated here is a method ...
متن کاملKnives of High Silica Content Glass for Thin-Sectioning
The use of glass knives in cutting sections for electron microscopy has been a standard procedure for several years. During this period various modifications in methods of scoring and breaking knives have been recommended, as well as types of glass which can be used (1, 2). Knives of high silica content glass were first suggested by Eaves and Flewett (1953) who reported that such knives retaine...
متن کاملA petrographic thin sectioning technique for evaluating composite materials
The development of tough high-peformance composites often employs a variety of analytical methods that correlate morphological, yield and fracture characteristics to the corresponding properties of a composite. Most of these techniques can be severely limited due to the high concentration of fibres used in high performance composites (nominally 60 wt %). Several light microscopy techniques can ...
متن کاملShape reconstruction techniques for optical sectioning of arbitrary objects
This paper considers the reconstruction of a shape from an object’s optical sectioning. To this end we describe three algorithms relying on the scalar optics model of light propagation of which the most involved is the deconvolution approach. This approach produces a sequence of deblurred images. We improve the deconvolution approach by suggesting a novel stopping criterion for the iterative pr...
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ژورنال
عنوان ژورنال: The Journal of Biophysical and Biochemical Cytology
سال: 1957
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.3.2.311